A cantilever with a sharp tip is excited in resonance by positive feedback,
controling a constant amplitude A. For the free cantilever, the eigenfrequency is
given by f0 = 2π ( k / m )0.5, k is the spring
constant and m is the effective mass of the cantilever. Forces between tip and sample change
the frequency f = 2π ( k + kts /m)0.5, where the
tip-sample force gradient is given by kts. When kts is small compared
to k and essentially constant within the tips trajectory to and from the sample, a frequency change
results. Forces cause a frequency shift, and with a feedback circuit adjusting the sample height
such that Δf remains constant, an image is created.
"Classic" frequency-modulation-AFM with cantilevers with small spring stiffness k ≈ 20 N/m and
comparatively large amplitudes A ≈ 10 nm enables routine-imaging at atomic resolution.
However, theory shows that resolution enhancement is possible by using sub-nm amplitudes with stiff cantilevers.
Minimal image noise δz is then proportional to
where λ is the range of the tip-sample forces. For chemical forces, λ ≈ 0.1 nm. Optimal
resolution is expected for A ≈ λ.
Why didn't people use small amplitudes from the beginning?
Operation with sub-nm amplitudes is thus only possible using very stiff cantilevers (k ≈ 1 kN/m). Traditional silicon cantilevers with such a large stiffness are usually not available, moreover they suffer from two additional disadvantages:
A solutions to these problems offers the qPlus Sensor design.